Figure 1

Two-dimensional gel of protein from SW480 colon adenocarcinoma cells. SW480 cells were cultured as described in Methods. In this representative case the cells were not treated with quercetin and were thus a control. Cells were harvested; protein was extracted and subjected to two-dimensional electrophoresis as described in Methods. The gel was stained with Coomassie blue. The protein spots indicated were those subsequently determined to be significant in the effects of quercetin.